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cleancap® reagent au n-7114-10 ![]() Cleancap® Reagent Au N 7114 10, supplied by TriLink, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/cleancap® reagent au n-7114-10/product/TriLink Average 90 stars, based on 1 article reviews
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Image Search Results
Journal: iScience
Article Title: Controllable self-replicating RNA vaccine delivered intradermally elicits predominantly cellular immunity
doi: 10.1016/j.isci.2023.106335
Figure Lengend Snippet: In vivo expression of LUC after intradermal injection of c-srRNA (A) Schematic diagram of c-srRNA structure. Non-structural proteins (nsP1-nsP4) encode a replica of Venezuelan equine encephalitis virus (VEEV). c-srRNA is produced by in vitro transcription and has a form of mRNA with 5′-Cap and 3′-polyA sequence. No modified nucleosides. (B–D) mRNAs, mRNA-LUC (CleanCap Fluc mRNA [5-methoxyuridine], Tri-Link L-7202) or c-srRNA1-LUC, were prepared as naked RNAs, without LNP or other transfection reagents, in lactated Ringer’s solution. Five microgramsof mRNAs was injected intradermally into a single site on the right hind limb of CD-1 outbred mice (Day 0). Luciferase activity was visualized and quantitated by using a bioluminescent imaging system, AMI HTX (Spectral Instruments Imaging, Tucson, AZ) from Day 1 through Day 26. (B) Representative pictures of mice that received the intradermal injection of mRNA-LUC. (C) Representative pictures of mice that received the intradermal injection of c-srRNA1-LUC. (D) Changes in LUC activities over time for the mice are shown in (B) and (C). Luciferase activity was represented as average radiance.
Article Snippet:
Techniques: In Vivo, Expressing, Injection, Virus, Produced, In Vitro, Sequencing, Modification, Transfection, Luciferase, Activity Assay, Imaging
Journal: iScience
Article Title: Controllable self-replicating RNA vaccine delivered intradermally elicits predominantly cellular immunity
doi: 10.1016/j.isci.2023.106335
Figure Lengend Snippet:
Article Snippet:
Techniques: Virus, Recombinant, Modification, Derivative Assay, Variant Assay, Saline, Plasmid Preparation, Enzyme-linked Immunospot, Double-Color Enzymatic ELISPOT, Enzyme-linked Immunosorbent Assay, Plaque Reduction Neutralization Test, Software, Imaging